Gibco™ McCoy's 5A Medium (Park and Terasaki modification)

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

Brand:  Gibco™ 80014020

Product Code. 11574526

Description

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A (modified) is provided as follows:.

With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red.

Without: Sodium Pyruvate.

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. Park and Terasaki modified McCoy's medium with modified levels of glycine, L-glutamic acid, L-hydroxyproline, L-proline, L-serine, L-threonine, L-tryptophan, and L-valine, plus the addition of antibiotics and HEPES.

Product Use.

For Research Use Only. Not for use in diagnostic procedures.

Gibco™ McCoy's 5A (Park and Terasaki modification) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (Park and Terasaki modification) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Content And Storage: Storage conditions:2°C to 8°C. Protect from light.
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Form: Liquid
• Sterility: Sterile-filtered
• Without Additives: No Sodium Pyruvate
• Quantity: 1 Ea.

• Concentration: 1 X
• Product Type: McCoy's 5A Medium
• With Additives: High Glucose, Glutamine, HEPES, Phenol Red
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard