Gibco™ McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

Brand:  Gibco™ 36600021

Product Code. 11594466

Description

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

With: High Glucose, GlutaMAX™, Bacto-peptone, Phenol Red

Without: Sodium Pyruvate

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO₂ incubator.

Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light.
  Shipping conditions: Ambient.
  Shelf life: 12 months from date of manufacture.
• Cell Type: Mammalian Cells
• Concentration: 1 X
• Form: Liquid
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Sodium Pyruvate
• Osmolality: 280 - 320 mOsm/kg
• Product Line: Gibco™, GlutaMAX™
• Shipping Condition: Room Temperature

• Culture Environment: CO₂
• Classification: Animal Origin-free
• Culture Type: Mammalian Cell Culture
• Product Type: McCoy's 5A Medium
• With Additives: High Glucose, GlutaMAX, Phenol Red, Bacto-peptone, Sodium Bicarbonate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• pH: 6.9 to 7.1
• Quantity: 500 mL