Invitrogen™ MCP-5 (CCL12) Mouse ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15475223	Invitrogen™ EMCCL12	96 Tests
17125689	Invitrogen™ EMCCL12X5	5 x 96 Tests

Description

The Mouse MCP-5 (CCL12) ELISA quantitates Ms MCP-5 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MCP-5. Principle of the method The Mouse MCP-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

Specifications

• Accession Number: Q62401
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 20293
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Angiogenesis
• Synonym: MCP-5, Scya12
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 0.5 pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: MCP-5,Scya12
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.