Invitrogen™ MEGAscript™ SP6 Transcription Kit

Synthesize 10 to 50 times the amount of RNA produced by conventional transcription reactions

Brand:  Invitrogen™ AM1330M

Product Code. 10418644

Description

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions. Each Ambion kit includes sufficient reagents for 40 reactions.

• Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
• Amplifies aRNA for gene array analysis and other applications
• Efficiently incorporates many modified nucleotides
• A typical 20μL MEGAscript reaction with 1μg of the pTRI-Xef-1α control template will yield over 100μg of transcript
• Versatility of the MEGAscript kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase
• Accessory Products:
• MessageAmp™ aRNA Amplification Kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr. No. AM1908) and the MEGAclear-96 Kit (Mfr. No. AM1909).

Specifications

• Content And Storage: SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at -20°C. Nuclease-free Water may be stored at any temperature.
• Includes: SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water
• Product Type: Kit with manual

• For Use With (Application): Gene Expression Analysis and Genotyping, in vitro Transcription
• No. of Reactions: 40

For Research Use Only. Not for use in diagnostic procedures.