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MHC I Free Chain, APC, clone: A4, eBioscience™
Mouse Monoclonal Antibody
Brand: Invitrogen 17-9958-41
This item is not returnable.
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Description
There are three class I alpha-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with B2 microglobulin (B2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells.
Description: The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of beta2 microglobulin. There are three class I alpha-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with beta2 microglobulin (beta2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells.Applications Reported: This A4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This A4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
MHC I Free Chain | |
Monoclonal | |
5μl/Test | |
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
Mouse | |
25 Tests | |
Primary | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Liquid |
Flow Cytometry | |
A4 | |
APC | |
MHC Free, MHC Class I free chain without beta 2 microglobulin, MHC Class I free chain, MHC Class 1 free chain | |
Affinity chromatography | |
RUO | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only.