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MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), FITC, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 11-5999-81
Description
Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin. Applications Reported: 28-14-8 has been reported for use in flow cytometric analysis. Applications Tested: The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.
This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.Specifications
MHC Class I (H-2Db) | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P01895, P01896, P01897, P01899, P01900, P14426, P14427 | |
H2-D1 | |
Affinity chromatography | |
RUO | |
14964 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
28-14-8 | |
FITC | |
H2-D1 | |
H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein | |
Mouse | |
50 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.