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Invitrogen™ MIP-1b (CCL4) Human Instant ELISA™ Kit
For the quantitative detection of MIP-1beta levels in cell culture supernatants, serum and plasma.
£487.00 - £2845.00
Specifications
Accession Number | P13236 |
---|---|
Assay Range | 7.8-500 pg/mL |
Assay Sensitivity | 3.0 pg/mL |
Conjugate | Biotin |
Product Type | ELISA Kit |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15551527
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Invitrogen™
BMS2030INST |
128 Tests |
£487.00
128 tests |
Estimated Shipment: 11-11-2024
Log in to see stock. |
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15561527
|
Invitrogen™
BMS2030INSTTEN |
10 x 128 Tests |
£2845.00
Pack of 10 |
Estimated Shipment: 29-10-2024
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Description
The Human Macrophage Inflammatory Protein-1beta (CCL4)(Hu MIP-1β) ELISA quantitates Hu MIP-1β in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu MIP-1β. Principle of the method The Human MIP-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Pre-coated plate(s)Specifications
P13236 | |
3.0 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
4.9% | |
Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers | |
RUO | |
-20°C | |
Human | |
3 hr. 10 min. |
7.8-500 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
6351 | |
7.2% | |
HRP | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
CCL4, LAG-1, Small inducible cytokine A4, SCYA4 | |
20 min. |
For Research Use Only