Invitrogen™ CellTracker™ Fluorescent Probes

CellTracker™ is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements.

Product Code	Brand	Dye Type	Quantity
11564237	Invitrogen™ C2110	CellTracker™ Blue CMAC	5 mg
11574237	Invitrogen™ C2111	CellTracker™ Blue CMHC	5 mg
11584217	Invitrogen™ C12881	CellTracker™ Blue CMF2HC	5 mg
15352644	Invitrogen™ C34565	CellTracker™ Deep Red	20 x 15 μg
11570166	Invitrogen™ C7025	CellTracker™ Green CMFDA	20 x 50 μg
10644013	Invitrogen™ C2925	CellTracker™ Green CMFDA	1 mg
11554237	Invitrogen™ C2102	CellTracker™ Green BODIPY™	5 mg
11504267	Invitrogen™ C34551	CellTracker™ Orange CMRA	20 x 50 μg
10082742	Invitrogen™ C2927	CellTracker™ Orange CMTMR	1 mg
10724274	Invitrogen™ C10094	CellTracker™ Violet BMQC	5 x 0.1 mg

Description

Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

Features of the CellTracker dyes include:

• Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells
• Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations)
• Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion
• Low cytotoxicity—does not affect viability or proliferation
• Cell Versatility– works with cells in suspension and adherent cells

The CellTracker fluorescent probes have been designed to freely pass through cell membranes; however, once inside the cell are transformed into cell-impermeant reaction products. The CellTracker fluorescence probes (except for CellTracker Deep Red) contain a chloromethyl or bromomethyl group that reacts with thiol groups, utilizing a glutathione S-transferase–mediated reaction. In most cells, glutathione levels are high (up to 10 mM) and glutathione transferase is ubiquitous. CellTracker Deep Red probe contains a succinimidyl ester reactive group, which reacts with amine groups present on proteins.

After conversion to impermeant versions, the CellTracker fluorescent probes are well retained in living cells through several generations. The probes are transferred to daughter cells, but are not transferred to adjacent cells in a population. Cells loaded with the CellTracker fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, several CellTracker fluorescent probes with various excitation and emission spectra are available allowing for multiplexing in fluorescence imaging. The CellTracker dyes are retained with fixation and permeabilization, enabling their use with antibodies for fluorescence immunostaining applications.

Spectral characteristics of the fluorescent CellTracker probes:

• CellTracker Blue CMAC—353/466 nm
• CellTracker Blue CMF2HC—371/464 nm
• CellTracker Blue CMHC—372/470 nm
• CellTracker Violet BMQC—415/516 nm
• CellTracker Green CMFDA—492/517 nm
• CellTracker Green BODIPY™—522/529 nm
• CellTracker Orange CMTMR—541/565 nm
• CellTracker Orange CMR—548/576 nm
• CellTracker Red CMTPX—577/602 nm
• CellTracker Deep Red—630/660 nm

Fluorescent CellTracker reagents include the blue-fluorescent chloromethyl derivatives of amino-, hydroxy-, and difluorohydroxycoumarin (CMAC, CMHC and CMF2HC), the green-fluorescent chloromethyl derivatives of fluorescein diacetate (CMFDA) and a BODIPY™ dye, the orange-fluorescent CMTMR and CMRA, and the red-fluorescent CMTPX. CellTracker Blue CMAC, CMHC, and CMF2HC, CellTracker Violet, the violet-fluorescent bromomethyl derivative of coumarin (BMQC), CellTracker Green BODIPY™, CellTracker Orange CMTMR, and CellTracker Red CMTPX do not require enzymatic cleavage to activate their fluorescence, whereas the green CMFDA and orange CMRA do require enzymatic cleavage. The impermeable reaction products of the chloromethyl or bromomethyl coumarins have excellent retention, strong fluorescence, and relatively uniform cytoplasmic staining, making these derivatives potentially useful for correcting motion artifacts in imaging. CMFDA is colorless and non-fluorescent until cytosolic esterases cleave off the acetates, releasing a brightly fluorescent product.

In addition to bright fluorescence and excellent retention, the CellTracker fluorescent probes do not contribute to cytotoxicity.

Order Info

Shipping Condition: Room Temperature

Specifications

• Form: Dry Powder
• Label Type: Other Labels or Dyes
• Product Type: Dye

For Research Use Only. Not for use in diagnostic procedures.