Invitrogen™ Di-4-ANEPPDHQ

Molecules that fluoresce in response to electrical potential changes in their environment

Brand:  Invitrogen™ D36802

Product Code. 10493103

Description

Increases and decreases in membrane potential-referred to as membrane hyperpolarization and depolarization, respectively-play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, cell signaling and ion-channel gating. Potentiometric probes are important tools for studying these processes.

• Fast-response probes that operate by means of change in their electronic structure, and consequently their fluorescence properties, in response to change in surrounding electric field
• Optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains
• Magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show 2-10% fluorescence change per 100mV
• Display potential-dependent shift in their excitation spectra, thus permitting quantitation of membrane potential using excitation ratio measurements
• Cationic molecule; exhibits very low internalization and good signal:noise ratio and is useful for neural network analysis
• Ex/Em maxima bound to model phospholipid membranes are approximately 465/635nm (but spectral properties are highly dependent on environment)
• Nonfluorescent until bound to membranes
• Soluble in ethanol, DMSO, and DM
• Dye is introduced into cells by direct addition of stock solution to cell culture medium, by using by retrograde labeling
• Plasma membrane of cell has transmembrane potential of approximately -70mV (negative inside) as a consequence of K+, Na+, and Cl- concentration gradients that are maintained by active transport processes
• Potentiometric probes offer indirect method of detecting the translocation of these ions

Cell Analysis, Cell Metabolism, Cell Structure, Cell Viability, Proliferation and Function, Membranes (General) and Lipids

Order Info

Shipping Condition: Room temperature

Specifications

• Color: Infrared
• Detection Method: Fluorescence
• Product Type: ANEP Dye
• Shipping Condition: Room Temperature

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• For Use With (Equipment): Fluorescence Microscope
• Quantity: 1 mg
• Sub Cellular Localization: Cytoplasm & Cytosol

For Research Use Only. Not for use in diagnostic procedures.