Invitrogen™ EnzChek™ Ultra Amylase Assay Kit

Measures amylase activity or screens amylase inhibitors in a high-throughput format

Brand:  Invitrogen™ E33651

Product Code. 10093552

Description

The EnzChek Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

• Label (Ex/Em): BODIPY FL conjugate (approx. 502/512nm)
• Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
• Sufficient reagents are supplied for 500 assays (using a 100μL assay volume in a 96-well microplate assay format)

Find Fluorescent Substrates for Other Glycosidases
In addition to the EnzChek Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more.

Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, High-Content Screening (HCS), Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store at room temperature and protect from light.
• For Use With (Application): Ultra Amylase Assay
• Product Type: Amylase Assay
• Quantity: 1 Kit
• Substrate Properties: Molecular Substrate
• Target Enzyme: Amylase

• Detection Method: Fluorescence Intensity
• For Use With (Equipment): Fluorescence Microplate Reader
• Product Line: EnzChek™
• Shipping Condition: Room Temperature
• Substrate Type: Amylase Substrate

For Research Use Only. Not for use in diagnostic procedures.