Invitrogen™ EnzChek™ Lysozyme Assay Kit

Measure lysozyme activity in solution

Brand:  Invitrogen™ E22013

Product Code. 10565393

Description

The EnzChek Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The increase in fluorescence resulting from lysozyme activity is measured using a fluorometer or fluorescence microplate reader. The assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.

• Detect lysozyme activity concentrations as low as 20U/mL
• Use standard fluorescein (FITC) excitation/emission settings
• Format allows for continuous measurement of lysozyme activity

Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, High-Content Screening (HCS), Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• For Use With (Application): Lysozyme Assay
• Product Type: Lysozyme Assay
• Quantity: 400 assays
• Substrate Properties: Lipid-Based Substrate
• Target Enzyme: Lysozyme

• Detection Method: Fluorescence Intensity
• For Use With (Equipment): Fluorescence Microplate Reader
• Product Line: EnzChek™
• Shipping Condition: Room Temperature
• Substrate Type: Lysozyme Substrate

For Research Use Only. Not for use in diagnostic procedures.