Invitrogen™ EnzChek™ Phosphatase Assay Kit

Continuous assay of prostatic acid phosphatase and protein phosphatase 1

Brand:  Invitrogen™ E12020

Product Code. 10338352

Description

Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes (proprietary) 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate. DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∽1000 assays, using a reaction volume of 100μL per assay. Fluorescence (excitation/emission maxima approx. 358/455nm) can be measured in a fluorescence microplate reader or a standard fluorometer.

Cell Analysis, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

Specifications

• Color: Blue
• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Dye Type: DiFMU
• Product Line: EnzChek™
• Shipping Condition: Room Temperature
• Substrate Properties: Chemical Substrate
• Target Enzyme: Phosphatases

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• For Use With (Application): Phosphatase Assay
• Product Type: Phosphatase Assay
• Emission: 360⁄460
• Quantity: 1000 Assays
• Substrate: DiFMUP
• Substrate Type: Phosphatase Substrate

Safety and Handling

Product Identifier

• EnzChek Phosphatase Assay Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Reproductive toxicity Category 1B

Hazard Statement

• H226-Flammable liquid and vapour.
• H312-Harmful in contact with skin.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H360D-May damage the unborn child.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P233-Keep container tightly closed.
• P240-Ground/bond container and receiving equipment.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P242-Use only non-sparking tools.
• P243-Take precautionary measures against static discharge.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Dimethylformamide

For Research Use Only. Not for use in diagnostic procedures.