Invitrogen™ H2DCFDA (H2-DCF, DCF)

Chemically reduced form of fluorescein used as an indicator for reactive oxygen species in cells

Brand:  Invitrogen™ D399

Product Code. 11560326

Description

The cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H₂DCFDA) (also known as dichlorofluores cin diacetate) detects reactive oxygen species (ROS) in cells, for example to detect generation of reactive oxygen intermediates in neutrophils and macrophages. Upon cleavage of the acetate groups by intracellular esterases and oxidation, nonfluorescent H₂DCFDA is converted to highly fluorescent 2',7'-dichlorofluorescein (DCF).

• Ex/Em: ∽492 - 495/517 - 527nm
• Product is air sensitive (store under dry argon or nitrogen)
• Product may be dissolved in DMSO, DMF, or ethanol for use
• Indicator is cell permeant
• Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein

Refer to the Molecular Probes™ Handbook for additional product information.

Apoptosis, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Free Radical Detection, Nitro-Oxidative Stress, Reactive Oxygen Species

Order Info

Shipping Condition: Room Temperature

Specifications

• Quantity: 100 mg

For Research Use Only. Not for use in diagnostic procedures.