Invitrogen™ Low Density Lipoprotein from Human Plasma, BODIPY™ FL complex (BODIPY™ FL LDL)

Nonacetylated conjugate

Brand:  Invitrogen™ L3483

Product Code. 10307932

Description

Human low-density lipoprotein (LDL) is large protein complex (approx. 500,000 Da) that binds to a specific receptor on the surface of vertebrate cells and delivers cholesterol via receptor-mediated endocytosis. Experiments to study LDL are typically performed by adding fluorescently labeled LDL to cultured cells and analyzing them by microscopy or flow cytometry. Alternatively the fluorescently labeled LDL can be injected into test animals, and the distribution of label can be analyzed after specified time period.

• Label (Ex/Em): BODIPY FL (515/520)
• Acetylated: No
• Amount: 200μL (1.0mg/mL)
• Prepared from fresh human plasma approximately every two months
• Key Applications: Counting cell-surface LDL receptors, and analyzing their motion and clustering following internalization; Quantitating LDL receptor activity in fibroblasts (replacing the radiolabeled LDL assay); Investigating LDL expression and identifying LDL receptor deficiencies in various cell lines

Cell Analysis, Cell Cycle, Cell Structure, Cell Viability, Proliferation and Function, Endocytosis, Exocytosis and Phagocytosis, Lysosomes, Vacuoles

Order Info

Shipping Condition: Wet Ice

Specifications

• Concentration: 1 mg⁄ml
• Detection Method: Fluorescence
• Dye Type: BODIPY Dyes
• Product Line: BODIPY™
• Shipping Condition: Wet Ice

• Content And Storage: Store in refrigerator (2–8°C) and protect from light.
• Product Type: Supplement
• Form: Liquid
• Quantity: 200 μL

For Research Use Only. Not for use in diagnostic procedures.