Invitrogen™ LysoTracker™ Deep Red

Deep red-fluorescent dye for labeling and tracking acidic organelles in live cells

Brand:  Invitrogen™ L12492

Product Code. 15107482

Description

LysoTracker Deep Red is a cell-permeable, non-fixable, red fluorescent dye that stains acidic compartments within a cell, such as lysosomes. Deep Red has an excitation and emission maximum of 647/668 nm and can be efficiently excited using a Cy5 filter. LysoTracker probes are available in a variety of fluorescent colors.

Simple, highly specific, one-step staining and tracking of acidic organelles
LysoTracker probes consist of a hydrophobic fluorophore linked to a weak base that is only partially protonated at neutral pH. In a neutrally charge state, LysoTracker probes can freely diffuse across intact plasma membranes of live cells. Due to the inherently acidic properties within the lysosome, upon diffusion into the lysosome the weakly basic moiety is protonated. In this charged state, the probe does not readily diffuse across the organelle membrane, providing a localized accumulation for distinct staining of acidic organelles such as lysosomes. Effective at nanomolar concentrations, LysoTracker probes are highly selective for acidic organelles and provide simple one-step staining that does not rely on antibody detection.

Lysosomes are acidic in nature due to the production and storage of digestive enzymes (hydrolases). The acidic environment of lysosomes enables degradation of carbohydrates, lipids, nucleic acids, and peptides. Extracellular proteins, virus or bacteria can also be internalized and trafficked to the lysosome for degradation via the lysosomal proteolysis pathway. In addition, misfolded proteins or damaged organelles become targets for lysosomal degradation via the induction of autophagy. Autophagy is important for cellular differentiation, survival during nutrient deprivation, and normal growth control. The inherent acidic nature within lysosomes, and any change of pH during biosynthesis or pathogenesis, can be exploited by various probes that respond to an acidic environment. LysoTracker products provide fluorescence detection for live-cell staining of acidic environments and can be used for labeling and tracing acidic organelles such as lysosomes.

Specifications

• Color: Far-Red
• Content And Storage: Store desiccated at ≤–20°C
  • Protect from light
  • Avoid freeze-thaw cycles and do not store in a frost-free freezer
  • Store in single-use aliquots, if possible
  
  
• For Use With (Equipment): Fluorescence Microscope, Fluorescent Imager
• Detection Method: Fluorescence
• Emission: Near IR
• Product Line: LysoTracker™
• Label Type: Fluorescent Dye
• SubCellular Localization: Lysosomes

• Concentration: 1 mM
• Excitation Wavelength Range: 647/668
• Quantity: 5 x 50 μL
• Description: LysoTracker™ Deep Red
• Form: Liquid
• Shipping Condition: Room Temperature
• Product Type: Dye

Safety and Handling

Product Identifier

• LysoTracker Deep Red *1 mM solution in DMSO*

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : DMSO

For Research Use Only. Not for use in diagnostic procedures.