Invitrogen™ Qtracker™ 625 Cell Labeling Kit

Use custom targeting peptide to deliver orange/red-fluorescent nanocrystals into cytoplasm of live cells

Brand:  Invitrogen™ A10198

Product Code. 10638883

Includes: 100μL Qtracker nanocrystals, Component A (2μM in 50mm borate buffer, pH 8.3) and 100μL Qtracker carrier, Component B (phosphate buffered saline, pH 7.2).

Description

• Qtracker 625 label has Ex/Em 405-585nm/625nm
• Cytoplasmic delivery is not mediated by specific enzyme; therefore, no cell-type specificity has been observed
• Delivery is typically accomplished in less than 1 hour
• Qdot nanocrystals delivered are compatible with serum-sensitive cells
• Intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity
• Observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes
• Distributed in vesicles in cytoplasm, and are inherited by daughter cells for at least six generations: Fluorescence from the Qtracker labels can be seen up to a week after delivery in some cell lines
• Long-term cellular retention makes kits suitable for studying cell motility, migration, differentiation, morphology, and many other cellular function studies
• Labels do not leak out of intact cells to be taken up by adjacent cells in population
• Can be easily monitored on variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems
• Cytotoxicity of the materials use in Qtracker Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells: Labeling with Qtracker Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility
• Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics

Cell Analysis, Cell Tracing and Tracking, Cell Viability, Proliferation and Function, Chemotaxis and Cell Migration

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Contains 100 μl Qtracker™ nanocrystals, Component A (2 μM in 50 mM borate buffer, pH 8.3) and 100 μl Qtracker™ carrier,Component B (phosphate buffered saline, pH 7.2). Store at 2–6°C. Do not freeze. Stable for at least 6 months.
• Product Line: Molecular Probes™, QTRACKER™
• Shipping Condition: Room Temperature

• Quantity: 1 Kit
• Reagent Type: Cell Labeling Reagents
• Product Type: Cell Labeling

For Research Use Only. Not for use in diagnostic procedures.