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Invitrogen™ Qtracker™ 565 Cell Labeling Kit
Designed for loading cells grown in culture with highly fluorescent Qdot nanocrystals
Brand: Invitrogen™ Q25031MP
665.76 GBP valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Includes: 100μL Qtracker nanocrystals, Component A (2μM in 50mm borate buffer, pH 8.3) and 100μL Qtracker carrier, Component B (phosphate buffered saline, pH 7.2).
Description
- Use proprietary targeting peptide to deliver green-fluorescent Qdot 525 nanocrystals into cytoplasm of live cells
- Once inside cells, Qtracker labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in population
- Qtracker 525 label has Ex/Em (405-485/525)nm
- Provide intense, stable fluorescence that can be traced through several generations
- Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays
- Cytoplasmic delivery by this mechanism is not mediated by specific enzyme; therefore, no cell-type specificity has been observed
- Delivery is typically accomplished in less than 1 hour
- Compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity
- Observe labeled cells using extensive continuous illumination, without photobleaching and degradation problems often associated with organic dyes
- Distributed in vesicles in cytoplasm, and are inherited by daughter cells for at least six generations
- Can be seen up to a week after delivery in some cell lines
- Do not leak out of intact cells to be taken up by adjacent cells in population
- Qtracker reagent-labeled live cells can be easily monitored on variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems
- Cytotoxicity of materials has been tested in variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells: Labeling appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility
- Post-labeling, researchers have demonstrated wide variety of applications including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics
Cell Analysis, Cell Tracing and Tracking, Cellular Imaging, Immunofluorescence (IF)
Order Info
Shipping Condition: Room temperature
Specifications
Contains 100 μl Qtracker™ nanocrystals, Component A (2 μM in 50 mM borate buffer, pH 8.3) and 100 μl Qtracker™ carrier,Component B (phosphate buffered saline, pH 7.2). Store at 2–6°C. Do not freeze. Stable for at least 6 months. | |
QTRACKER™, Qdot™ | |
Room Temperature |
1 kit | |
Cell Labeling Reagents | |
Cell Labeling |
For Research Use Only. Not for use in diagnostic procedures.