Invitrogen™ RediPlate™ 96 EnzChek™ Serine/Threonine Phosphatase Assay Kit

Detects serine/threonine phosphatases and their corresponding modulators and inhibitors

Brand:  Invitrogen™ R33700

Product Code. 10145142

Description

The RediPlate 96 EnzChek Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensure that the assay is selective for Ser/Thr PPases- other phosphatases, including tyrosine phosphatase do not significantly react with the substrate. Additional advantages of this assay include compatibility with non-ionic detergents and insensitivity to free phosphate.

Drug Discovery and Development, Enzyme and Protein Activity Assays, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• For Use With (Application): Phosphatase Assay
• Product Type: Phosphatase Assay
• Emission: 358⁄452
• Shipping Condition: Room Temperature
• Substrate Properties: Chemical Substrate
• Target Enzyme: Serine⁄Threonine Phosphatases

• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Dye Type: DiFMU
• Product Line: EnzChek™, RediPlate™
• Substrate: DiFMUP
• Substrate Type: Phosphatase Substrate

For Research Use Only. Not for use in diagnostic procedures.