Invitrogen™ Rhodamine Phalloidin

High-affinity F-actin probe conjugated to red-orange fluorescent dye, tetramethylrhodamine (TRITC)

Brand:  Invitrogen™ R415

Product Code. 10063052

Description

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).

Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and Alexa Fluor conjugates including secondary antibodies.

• Selectively stains F-actin
• Excitiation/Emission: 540/565nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• Very widely cited fluorescent phalloidin conjugate

Cell Analysis, Cell Structure, Cytoskeleton

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Dye Type: TRITC (Tetramethylrhodamine Isothiocyanate)
• Label Type: Classic Dyes
• SubCellular Localization: Actin, Cytoskeleton

• Excitation Wavelength Range: 540⁄565
• Shipping Condition: Room Temperature
• Product Type: Phalloidin

Safety and Handling

Product Identifier

• Rhodamine Phalloidin

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 2

Hazard Statement

• H300-Fatal if swallowed.
• H310-Fatal in contact with skin.
• H330-Fatal if inhaled.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P262-Do not get in eyes, on skin, or on clothing.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : RHODAMINE Phalloidin

For Research Use Only. Not for use in diagnostic procedures.