Mouse anti-Human IgG (H+L) Secondary Antibody, Biotin, Invitrogen™

Mouse Polyclonal Secondary Antibody

Brand:  Invitrogen 31784

Product Code. 10351354

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31784 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31784 reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse, and mouse serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Reconstitute with 1 mL of distilled water. Country of Origin: USA

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG (H+L)
• Classification: Polyclonal
• Conjugate: Biotin
• Host Species: Mouse
• Quantity: 1 mL
• Primary or Secondary: Secondary
• Content And Storage: 4° C
• Isotype: IgG

• Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1.1 mg/mL
• Formulation: PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Human
• Form: Lyophilized