Invitrogen™ Mouse GM-CSF ProQuantum Immunoassay Kit

The ProQuantum Mouse GM-CSF Immunoassay Kit is designed to provide quantitative measurements of mouse GM-CSF in small sample volumes.

Brand:  Invitrogen™ A46735

Product Code. 17144303

Description

Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

• High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
• Broad dynamic range-5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
• Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
• Fast, easy workflow-2 hours from sample to answer, with no wash steps
• No proprietary instrument to purchase-runs on any real-time PCR instrument

GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).

Specifications

• Accession Number: colony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); colony-stimulating factor; CSF; CSF2; Csfgm; cytokine; GMCSF; Gm-CSf; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGC151255; MGC151257; MGI-IGM; M-GM-CSF; Molgramostin; put. GM-CSF; sargramostim
• Assay Sensitivity: <0.3 pg/mL
• For Use With (Equipment): qPCR Instruments
• Gene Symbol: CSF2
• Quantity: 96 Tests
• Sample Volume: Serum, 2-5 μL; Plasma, 2-5 μL; Supernatant, 2-5 μL
• Target: GM-CSF
• Total Assay Time: 20 hr

• Assay Range: 0.32 to 25,000 pg/mL
• Product Type: ELISA
• Gene ID (Entrez): P01587
• Kit Contents: Assay Dilution Buffer Antibody-Conjugate A Antibody-Conjugate B Antibody-Conjugate Dilution Buffer Standard Master Mix Ligase
• Regulatory Status: Research Use Only
• Storage Requirements: -20°C
• Test Time: 30 min