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Mouse IgG2a Isotype Control, Qdot™ 655, Invitrogen™
Mouse Isotype Control
Brand: Invitrogen Q10015
Code : 50
Additional Details : Weight : 0.50000kg
Description
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Mouse IgG2a Control antibody specifically detects Mouse IgG2a in Not Applicable samples. It is validated for Control, Flow Cytometry.Specifications
Mouse IgG2a | |
Qdot 655 | |
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2 | |
Mouse IgG2a | |
100 μL | |
Isotype Control (Primary) | |
4° C, store in dark | |
IgG2a |
Control, Flow Cytometry | |
50mM borate with 1M betaine and 0.05% sodium azide; pH 8.3 | |
Mouse | |
purified | |
RUO | |
Not Applicable | |
Liquid |