Proteintech™ Mouse IL-12 p70 ELISA Kit

K10014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL12 p70.

Brand:  Proteintech™ KE10014-96T

Product Code. 16851606

Description

• The assay recognizes mouse IL12 p70. An antibody specific for mouse IL12 p70 has been pre-coated onto the microwells.
• The mouse IL12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL12 p70 is added to detect the captured mouse IL12 p70 protein.
• For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
• Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
• Interleukin-12 (IL12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes.
• IL12 (referred to as 'p70') is a 70 kDa heterodimer composed of a 35 kDa subunit (IL12a p35) and a 40 kDa subunit (IL12b p40).
• IL12 is involved in the differentiation of native T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells.
• IL12 plays an important role in the activities of natural killer cells and T lymphocytes. IL12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.
• IL12 has been shown to play a critical role in the pathogenesis of a variety of immune-related diseases.

Specifications

• Accession Number: NP_032377 / NP_001290173
• Assay Sensitivity: 2.2 pg/mL
• Detection Method: Sandwich ELISA, Double Antibody
• Sample Type: Plasma, Serum
• Target Species: Mouse
• Host Species: Mouse
• Interassay CV: <10%
• Shipping Condition: Blue ice
• Total Assay Time: 4.5 hr.

• Assay Range: 15.6 to 1000 pg/mL
• Certifications/Compliance: ISO 13485
• Immunoassay Kit Format: Coated ELISA Kit
• Substrate: DAB
• Gene ID (Entrez): 16159
• Immunoassay Kit Grade: Solid
• Intraassay CV: <10%
• Storage Requirements: -20°C to +4°C
• UniProt ID: P43431 / P43432

Safety and Handling

Product Identifier

• 96TEST Mouse IL-12 p70 ELISA Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P234-Keep only in original container.
• P264-Wash skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P362-Take off contaminated clothing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine