Invitrogen™ IL-6 Mouse Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of mouse IL-6 protein levels from samples including serum, plasma, cell lysates and cell culture supernatants.

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Product Code	Brand	Quantity
13518930	Invitrogen™ 88-7064-22	2 x 96 Tests
15591027	Invitrogen™ 88-7064-76	20 x 96 Tests
15511037	Invitrogen™ 88-7064-86	10 x 96 Tests

Description

The Mouse Interleukin-6 (IL-6) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-6. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Mouse IL-6 ELISA Ready-SET-Go! Reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA)

Specifications

• Accession Number: P08505
• Assay Sensitivity: 4 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: HP40,IL-9,P40
• Label or Dye: HRP
• Sample Volume: Cell Lysate,100 μL; Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
• Synonym: Interleukin-6
• Test Time: 1 hr. 45 min.

• Assay Range: 4-500 pg/mL
• Conjugate: Biotin
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 16193
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 24 hr. 30 min.

For Research Use Only