Invitrogen™ TNF alpha Mouse Uncoated ELISA Kit with Plates

Engineered for accurate and precise measurement of mouse TNF alpha protein levels from samples including serum, plasma, tissue homogenate, lavage fluid and cell culture supernatants. Recognition of mouse TNF alpha is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.

Product Code	Brand	Quantity
11560637	Invitrogen™ 88-7324-22	2 x 96 Tests
15571107	Invitrogen™ 88-7324-76	20 x 96 Tests
15591107	Invitrogen™ 88-7324-86	10 x 96 Tests

Description

The Mouse Tumor Necrosis Factor Alpha (TNF-α) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse TNF-α. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Mouse TNF alpha ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA)

Specifications

• Accession Number: P06804
• Assay Sensitivity: 8 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CLMF,CLMF2,IL-12B,IMD28,IMD29,NKSF,NKSF2,CLMF,IL-12A,NFSK,NKSF1,P35
• Label or Dye: HRP
• Sample Volume: Lavage Fluid,100 μL; Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL; Tissue Homogenate,100 μL
• Synonym: Tumor Necrosis Factor alpha, tnf-a,tnf alpha
• Test Time: 1 hr. 45 min.

• Assay Range: 8-1,000 pg/mL
• Conjugate: Biotin
• Sample Type: Lavage Fluid, Plasma, Serum, Supernatant, Tissue Homogenates
• For Use With (Application): ELISA
• Gene ID (Entrez): 21926
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 24 hr. 30 min.

For Research Use Only