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Invitrogen™ TSLP Mouse Uncoated ELISA Kit with Plates
Engineered for the accurate and precise measurement of mouse TSLP in serum, and tissue culture supernatant samples.
Brand: Invitrogen™ 88-7490-22
115.33 GBP valid until 2024-09-28
Use promo code "21626" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21626” to get your promotional price
Includes:
- Capture Antibody: Pre-titrated, purified antibody
- Detection Antibody. Pre-titrated, biotin-conjugated antibody
- Standard. Recombinant cytokine for generating standard curve and calibrating samples
- Coating Buffer. 10X PBS ELISA Coating Buffer
- Assay Diluent. 5X Concentrated
- Detection Enzyme. Pre-titrated Avidin-HRP
- Substrate Solution. Tetramethylbenzidine (TMB) Solution
- Certificate of Analysis. Lot-specific instructions for the dilution of antibodies and standards
Description
The Mouse TSLP Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse TSLP. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.Specifications
Q9JIE6 | |
16 pg/mL | |
ELISA Kit | |
Mouse | |
Colorimetric Microplate Reader | |
IL-2,TCGF,lymphokine | |
HRP | |
RUO | |
2°C to 8°C | |
Mouse | |
24 hr. 30 min. |
16-2,000 pg/mL | |
Biotin | |
Serum, Supernatant | |
ELISA | |
53603 | |
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards, 96-Well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86) | |
2 x 96 Tests | |
Serum, 100 μL; Supernatant, 100 μL | |
Thymic Stromal-Derived Lymphopoietin | |
1 hr. 45 min. |
For Research Use Only