MP Biomedicals™ Gnome™ DNA Isolation Kit

Gnome™ DNA Isolation Kit offers a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types.

Product Code	Brand	No. of Reactions
11343139	MP Biomedicals™ 112010600	100 Preps
11333139	MP Biomedicals™ 112010400	25 Preps

Description

• No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.
• The GNOME™ kit utilizes RNase Mixx to eliminate RNA immediately after lysis, and Protease Mixx to rapidly degrade cellular proteins. This is followed by a proprietary ''salting-out'' technique which precludes the need for phenol, chloroform, or other organic extractions.
• The DNA can either be spooled, removed with forceps, or centrifuged and dissolved in TE.
• The GNOME™ DNA Isolation kit is used to quickly and efficiently isolate high molecular weight genomic DNA from cells and tissues of any type, including bacteria, yeast, plant, and animal cells.
• Each preparation with the GNOME™ kit yields up to 100 μg of genomic DNA.
• The DNA isolated by the GNOME™ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis.

Specifications

• Format: Kit
• Includes: Cell Lysis/Denaturing Solution, RNase Mixx, Protease Mixx, Salt Out Mixture, Cell Suspension Solution
• Product Type: Gnome™ DNA Isolation Kit
• Yield: 100 μg

• Sample Type: Blood, Tissues, Yeast, Bacteria, Plant
• pH: Cell Lysis/Denaturing Solution pH range: 6.2 to 6.9, Salt-out Mixture pH range: 6.0 to 6.8, Cell Suspension Solution pH range: 8.0 to 8.2
• Sample Volume: 20 to 50 mg wet weight bacterial cell pellet, Up to 250 mg plant tissue, 2 to 5 x 107 nucleated white blood cells, 50 to 250 mg mammalian tissue, ∼1 to 5 x 108 yeast cell pellet

Safety and Handling

Product Identifier

• gnome DNA isolation kit

Signal Word

• Warning

Hazard Category

• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Supplemental information

• MIXTURE LIST-Contains : sodium dodecyl sulphate

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.