N,N'-Bis(acryloyl)cystamine, Electrophoresis Reagent, 98%, Thermo Scientific Chemicals

Description

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Solubility
Soluble at 50 mg/ml in acetic acid

Notes
Store at -20°C. Incompatible with heat, oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 60984-57-8
• Molecular Formula: C10H16N2O2S2
• Molecular Weight (g/mol): 260.37
• MDL Number: MFCD00036225
• InChI Key: DJVKJGIZQFBFGS-UHFFFAOYSA-N
• Synonym: n,n'-bis acryloyl cystamine, n,n'-bisacrylylcystamine, 2-propenamide, n,n'-dithiodi-2,1-ethanediyl bis, 2,2'-bisacrylamino diethyldisulfide, bis-acrylylcystamine: bac, n,n'-dithiodi-2,1-ethanediyl bis acrylamide, n-2-2-prop-2-enamido ethyl disulfanyl ethyl prop-2-enamide, n-2-2-prop-2-enoylamino ethyldisulfanyl ethyl prop-2-enamide, acmc-20ak9p, n,n-bis-acrylylcystamine
• PubChem CID: 100602
• IUPAC Name: N-[2-[2-(prop-2-enoylamino)ethyldisulfanyl]ethyl]prop-2-enamide
• SMILES: C=CC(=O)NCCSSCCNC(=O)C=C

• Melting Point: 123°C to 124°C
• Color: White
• Quantity: 1 g
• Solubility Information: Soluble at 50mg/mL in acetic acid
• Formula Weight: 260.37
• Percent Purity: 98%
• Physical Form: Powder
• Chemical Name or Material: N,N'-Bis(acryloyl)cystamine, Electrophoresis Reagent

Safety and Handling

Product Identifier

• N,N'-Bis(acryloyl)cystamine, Electrophoresis Reagent

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : N,N-(Dithiodi-2,1-ethanediyl)bis(acrylamide)

RUO – Research Use Only