Neomycin Phosphotransferase II Monoclonal Antibody (4B4D1), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA515275

Product Code. 11335253

Description

MA5-15275 targets NPT in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15275 immunogen is purified recombinant fragment of NPT expressed in E. Coli.

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Specifications

• Antigen: Neomycin Phosphotransferase II
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites with 0.03% sodium azide
• Immunogen: Purified recombinant fragment of NPT expressed in E. Coli.
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: ELISA, Western Blot
• Clone: 4B4D1
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid