Nitro Blue Tetrazolium chloride, 98+%, Thermo Scientific Chemicals

Description

Intense blue dye used in histochemistry determinations

Intense blue dye used in histochemistry determinations.Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease. It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Further, it is used in immunology for detection of alkaline phosphatase. In addition to this, it acts as an oxidant.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Intense blue dye used in histochemistry determinations.Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease. It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Further, it is used in immunology for detection of alkaline phosphatase. In addition to this, it acts as an oxidant.

Solubility
Soluble in water, ethanol and methanol.

Notes
Light sensitive. Store in a cool place. Incompatible with strong oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 298-83-9
• Molecular Formula: C40H30Cl2N10O6
• Molecular Weight (g/mol): 817.644
• MDL Number: MFCD00012159
• InChI Key: FSVCQIDHPKZJSO-UHFFFAOYSA-L
• Synonym: Nitro BT; Nitrotetrazolium Blue chloride
• PubChem CID: 9281
• ChEBI: CHEBI:9505
• IUPAC Name: 2-[2-methoxy-4-[3-methoxy-4-[3-(4-nitrophenyl)-5-phenyltetrazol-2-ium-2-yl]phenyl]phenyl]-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium;dichloride
• SMILES: COC1=C(C=CC(=C1)C2=CC(=C(C=C2)[N+]3=NC(=NN3C4=CC=C(C=C4)[N+](=O)[O-])C5=CC=CC=C5)OC)[N+]6=NC(=NN6C7=CC=C(C=C7)[N+](=O)[O-])C8=CC=CC=C8.[Cl-].[Cl-]

• Melting Point: ∼190°C (decomposition)
• Beilstein: 4115923
• Solubility Information: Soluble in water,ethanol and methanol.
• Formula Weight: 817.65
• Percent Purity: 99%
• Sensitivity: Light sensitive
• Color: Yellow
• Quantity: 1 g
• Physical Form: Crystalline Powder
• Chemical Name or Material: Nitro Blue Tetrazolium chloride

Safety and Handling

Product Identifier

• Nitro Blue Tetrazolium chloride

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash face, hands and anyexposed skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.

Supplemental information

• MIXTURE LIST-Contains : Nitro Blue Tetrazolium chloride

RUO – Research Use Only