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Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

£48.37 - £148.00

Specifications

Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Membrane Compatibility PVDF, Nitrocellulose
Product Line Invitrogen™
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Run Time 10 minutes
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Products 2
Product Code Brand No. of Reactions Gel Compatibility Price Quantity & Availability  
Product Code Brand No. of Reactions Gel Compatibility Price Quantity & Availability  
16351358
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Invitrogen™
A44717
A standard kit will label 10 mini gels or 10 mini gel-sized membranes Mini Precast Protein Gels, Midi Precast Protein Gels
£48.37
10 reactions
In Stock
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16341358
missing translation for 'viewDocuments' Detalles de la promoción
Invitrogen™
A44449
A standard kit will label 40 mini gels or 40 mini gel-sized membranes Any Gel Type and Gel Chemistry
£148.00
40 reactions
In Stock
to see stock.
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Descripción

Descripción

  • Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
  • Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
  • Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
  • Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
  • Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
  • Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.
Especificaciones

Especificaciones

The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Invitrogen™
10 minutes
Protein Labeling Reagent
PVDF, Nitrocellulose
Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Dry Ice
Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
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Seguridad y manipulación

Seguridad y manipulación

missing translation for 'shelfLife' : At least 2 years from receipt

SDS
Documentos

Documentos

Certificados

For Research Use Only. Not for use in diagnostic procedures.

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