Invitrogen™ c-Myc (Total) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KHO2041

Product Code. 12023589

Includes: c-Myc antibody-coated 96-well plate, c-Myc Total standard, Standard diluent buffer, c-Myc Total detection antibody, Anti-rabbit IgG-HRP (horseradish peroxidase) (100X), HRP diluent, Wash buffer concentrate (25X), Stabilized chromagen (TMB), Stop solution, Plate covers, Detailed protocol with validation tests

Description

The Human c-Myc Total (Hu c-Myc) ELISA quantitates Hu c-Myc in human cell lysates. The assay will exclusively recognize both natural and recombinant Hu c-Myc, independed of phosphorylation state. Principle of the method The Human c-Myc solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: P01106
• Assay Sensitivity: <5 pg/mL
• Conjugate: HRP
• Sample Type: Cell Lysates
• Target Species: Human
• Gene ID (Entrez): 4609
• Incubation Time: 4 hr.
• Intraassay CV: 4.9%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,5 μL
• Target: Human
• Total Assay Time: 4 hr.

• Assay Range: 15.6-1000 pg/mL
• Concentration: 15.6 to 1000pg/mL
• Product Type: ELISA Kit
• Substrate Type: Transcription Factors
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: MRTLC,bHLHe39,c-Myc,MYC
• Interassay CV: 3.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: c-Myc (total)
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

For research use only. Not intended for human or animal therapeutic or diagnostic use.