Invitrogen™ TNFR1 (Soluble) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KAC1761

Product Code. 10511475

Includes: Pre-coated plate, standard, controls, antibody-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Human Tumor Necrosis Factor Receptor 1 (Hu TNFR1) ELISA quantitates Hu TNFR1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFR1. Principle of the method The Human TNFR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor necrosis factor receptor 1 (TNFR1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. This receptor plays an important role in mediating apoptosis. TNFR1 also plays an important role in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of the receptor is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12.

Order Info

Shipping Condition: Dry Ice

Specifications

• Accession Number: P19438
• Assay Sensitivity: 50 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD120a,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR1,TNFR55,TNFR60,p55,p55-R,p60
• Interassay CV: 7.3%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
• Marker Gene: TNF-RI
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 1-47 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Colorimetric Detection
• Gene ID (Entrez): 7132
• Incubation Time: 1.25 hr.
• Intraassay CV: 4.6%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Target: Human
• Total Assay Time: 1 hr. 15 min.

For Research Use Only. Not for use in diagnostic procedures.