Invitrogen™ IL1RA Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KAC1181

Product Code. 10610354

Includes: Pre-coated plate, standard, controls, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Human IL-1RA ELISA Kit quantifies human Interleukin 1 Receptor Antagonist (IL-1RA) in serum, plasma, buffered solution, urine or cell culture medium. The assay will recognize both the natural and recombinant IL-1RA. Principle of the method The Human IL-1RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 1 receptor antagonist (ILRA) is a protein related to the IL-1 family based on its similarities in amino acid sequence to both IL-1 beta and IL-1 alpha, similarities in gene structure, and collective gene localization. The human gene contains four exons and maps to chromosome 2q13-14. IL-1 RA binds to the IL-1 receptor and blocks IL-1 action through competitive inhibition. In contrast to IL-1, the binding of IL-1RA does not initiate signaling events. The secretion of IL-1 RA is mediated by a classical signal sequence while neither IL-1alpha nor IL-1beta possess such a sequence. The lack of homology between IL-1 RA and IL-1 alpha or IL-1 beta near the N terminus may provide an explanation for the biosynthetic and functional differences between IL-1 RA and the two IL-1 proteins. The production of IL-1RA is stimulated by many substances including adherent IgG, cytokines, and bacterial or viral components. IL-1RA is an important natural anti-inflammatory protein that when missing or just available in inadequate amounts plays a key role in several pathological conditions such as rheumatoid arthritis, colitis, osteomyelitis, periostitis and pustulosis.

Order Info

Shipping Condition: Wet ice

Specifications

• Accession Number: P18510
• Assay Sensitivity: 4 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: DIRA,ICIL-1RA,IL-1RN,IL-1ra,IL-1ra3,IL1F3,IL1RA,IRAP,MVCD4
• Intraassay CV: 4.5%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL; Urine,10 μL
• Target: Human
• Total Assay Time: 3 hr. 30 min.

• Assay Range: 31.2-2000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant, Urine
• For Use With (Application): Colorimetric
• Gene ID (Entrez): 3557
• Interassay CV: 6.1%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Controls
• Precision: 4pg/mL
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

For Research Use Only. Not for use in diagnostic procedures.