Invitrogen™ IL-6 Human ELISA Kit

Key cytokine with actions in immune response

Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Product Code	Brand	Quantity
10334403	Invitrogen™ KHC0061	96 Tests
10484943	Invitrogen™ KHC0062	2 x 96 Tests
10571855	Invitrogen™ KHC0061C	5 x 96 Tests

Description

The Human Interleukin 6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The IL-6 Human ELISA Kit quantifies natural and recombinant human IL-6 in human serum, human plasma, buffered solution, or cell culture medium.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Wet ice

Specifications

• Accession Number: P05231
• Assay Sensitivity: <2 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
• Intraassay CV: 6.2%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 7.8-2500 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Colorimetric
• Gene ID (Entrez): 3569
• Interassay CV: 7.9%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Precision: <2pg/mL
• Sample Volume: Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
• Target: Human
• Total Assay Time: 3 hr.

For Research Use Only. Not for use in diagnostic procedures.