Invitrogen™ Recombinant Protein G Agarose

For the purification of IgG from most mammalian species and does not bind IgM, IgA, IgE or serum albumin

Brand:  Invitrogen™ 15920010

Product Code. 11598896

Includes: rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol.

Description

• Recombinant Protein G (rProtein G) Agarose is a ready-to-use affinity chromatography matrix supplied as a 50% slurry (in phosphate buffered saline (PBS), pH 7.0; 20% ethanol)
• The rProtein G is a recombinant form of streptococcal protein G engineered to eliminate nonspecific binding to serum albumin, and is immobilized on 4% agarose beads
• rProtein G Agarose has a binding capacity of approximately 20mg of human IgG permL of beads
• rProtein G Agarose binds to a broader range of IgG isotypes than react with protein A
• The binding characteristics and stability of rProtein G Agarose facilitate rapid and repeated use for affinity chromatography
• To purify IgG, load crude samples (serum, ascites fluid, or hybridoma supernatant) onto a column of rProtein G Agarose under physiologic conditions of salt and pH
• Non-IgG components do not bind to the beads and elute in the flow through fraction; Purified IgG is then eluted with a low pH buffer

PCR, PCR and Real-Time PCR

Order Info

Shipping Condition: Wet Ice

Specifications

• Column Type: Affinity Column
• Product Type: Agarose Gel
• Form: Suspension
• Stationary Phase: Protein G

• Content And Storage: rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol. Store at +4°C.
• Separation of: Protein
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.