Invitrogen™ p38 MAPK (Total) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KHO0061

Product Code. 10202643

Includes: p38 Antibody-Coated 96-Well Plate; p38 Total Standard; Standard Diluent Buffer; p38 Total Detection Antibody; anti-rabbit IgG-HRP (100X); HRP Diluent; Wash Buffer Concentrate (25X); Stabilized Chromogen, TMB; Stop Solution; Plate Covers; Detailed protocol with validation tests

Description

The Human p38 MAPK (Total) ELISA quantitates Hu p38 MAPK (Total) in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu p38 MAPK (Total). Principle of the method The Human p38 MAPK (Total) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: Q16539
• Assay Sensitivity: <16 pg/mL
• Conjugate: HRP
• Sample Type: Cell Lysates
• Target Species: Mouse, Rat
• Gene ID (Entrez): 1432
• Incubation Time: 4 hr.
• Intraassay CV: 5.1%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,10 μL
• Target: Human
• Total Assay Time: 4 hr.

• Assay Range: 31.2-2000 pg/mL
• Concentration: 31.2 to 2000pg/mL
• Product Type: ELISA Kit
• Substrate Type: Serine/Threonine Kinases
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CSBP,CSBP1,CSBP2,CSPB1,EXIP,Mxi2,PRKM14,PRKM15,RK,SAPK2A,p38,p38ALPHA
• Interassay CV: 7.3%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: p38
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

For Research Use Only. Not for use in diagnostic procedures.