Macherey-Nagel™ NTB Binding Buffer

Product Code	Brand	Quantity
11952282	Macherey-Nagel™ 740595.150	150 mL
12788482	Macherey-Nagel™ 740595.1	1000 mL

Description

NucleoTrap™ is a silica matrix based kit for isolation of highly pure DNA fragments from agarose gels and aqueous solutions (desalination). The kit can also be used for the removal of nucleotides and/or biotin after labelling reactions as well as for the removal of enzymes. By means of a specially activated spherical silica matrix it is possible to extract DNA fragments from agarose gels or solvents in a very mild manner, without the use of organic solvents. Nucleic acids are bound to the NucleoTrap™ matrix in the presence of a chaotropic salt. The hydrate shell of the nucleic acids is destroyed by this binding buffer, thus facilitating adsorption to the specially treated NucleoTrap™ matrix. A washing step is followed by the elution of DNA with low-salt elution buffer or distilled water. Kit components: NucleoTrap™ suspension, buffers.

• Silica matrix technology
• Fragment size: 20bp to 20kb
• Sample material: 20mg to 600mg agarose gel
• Elution volume: 20μL to 50μL
• Binding capacity: 6μg/10μL suspension
• Typical Recovery: 50% to 90%
• Preparation time: 60min/6 preps
• Format: silica bead suspension For extraction of DNA from agarose gels, suitable, for example, for subsequent cloning sequencing, PCR, restriction analysis. Support protocol for concentration and desalination of reaction mixtures.

Safety and Handling

Product Identifier

• buffer NTB

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H319-Causes serious eye irritation.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash with water thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.

Supplemental information

• MIXTURE LIST-Contains : guanidine hydrochloride, propan-2-ol