Macherey-Nagel™ Binding Buffer NTC

Cleans up single-stranded DNA. Macherey-Nagel™ Binding Buffer NTC is designed to obtain higher yields for small fragments using the NucleoSpin™ Gel and PCR Clean-Up Kit. It is used to purify short, single-stranded DNA and to extract small, single-stranded RNA from agarose gels.

Brand:  Macherey-Nagel™ 740654.100

Product Code. 11922312

Description

NucleoSpin™ Gel and PCR Clean-Up is a two-in-one kit with optimized recovery and elution volume for both PCR purification and gel extraction. The protocol ensures complete removal of contaminations for use in downstream applications, such as cloning, sequencing, PCR and restriction analysis.

Binding Buffer NTC is a supplemental buffer that obtains higher yields for small fragments compared to Buffer NTI, which is included in the kit. Generally, 200μL Buffer NTC is mixed with 100μL sample or 100mg agarose gel <2%. (Use 400μL NTC for 100mg agarose gel >2%.)

Applications:

• Clean-up/purification of short, single-stranded DNA
• Extract small, single-stranded RNA from agarose gels
• Achieve full recovery of fragments <50bp, even primer oligonucleotides

Specifications

• For Use With (Equipment): NucleoSpin™ Gel and PCR Clean-Up Kit
• Quantity: 100 mL
• For Use With (Application): For clean-up of single-stranded DNA and for Extraction of single-stranded RNA from agarose gel
• Buffer: Binding Buffer NTC

Safety and Handling

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H302-Harmful if swallowed.
• H312-Harmful in contact with skin.
• H332-Harmful if inhaled.
• H412-Harmful to aquatic life with long lasting effects.
• EUH031-Contact with acids liberates toxic gas.

Supplemental information

• MIXTURE LIST-Contains : potassium thiocyanate