Macherey-Nagel™ NucleoBond™ PC 10,000 Kit

Purifies up to 10mg of transfection-grade plasmid DNA from E. coli. Macherey-Nagel™ NucleoBond™ PC 10,000 Kit is a classic anion exchanger for fast and easy purification of high- and low-copy plasmids. Complete kit includes giga columns, buffers, RNase A and folder filters.

Brand:  Macherey-Nagel™ 740593

Product Code. 12877521

Description

NucleoBond™ kits utilize anion-exchange chromatography with optimized lysis buffer volumes for plasmid DNA purification. PC 10,000 kits are recommended for the isolation of high-copy plasmids (>20 copies/cell). Low-copy plasmids (<20 copies/cell) can be isolated as well, but this requires additional buffers.

• Folded Filters are included for fast and easy clarification of lysates with no centrifugation required and no shearing forces
• Contains sufficient buffer to perform 5 giga preps, with typical yields of 2,000–10,000μg of plasmid DNA from E. coli culture
• Columns are resistant to organic solvents and are free of DNase and RNase
• AX Resin can be used over a wide pH range (2.5–8.5) and can remain in contact with buffers for up to three hours

Specifications

• Buffer: Yes
• Isolation Technology: Anion Exchange Chromatography Technology
• For Use With (Application): Purification of high-copy and low-copy plasmids
• No. of Reactions: 5 Preps
• Quantity: 1 Kit
• Sample Volume: 500—2000mL (HC)
• Test Time: 120–150 min/2 preps

• Format: Gravity-flow Column (Giga)
• Sample Type: E. coli Culture
• Includes: Resuspension Buffer S1 (750mL), Lysis Buffer S2 (750mL), Neutralization Buffer S3 (750mL), Equilibration Buffer N2 (500/125mL), Wash Buffer N3 (1000/125mL), Elution Buffer N5 (500/125mL), RNase A lyophilized (75mg), NucleoBond AX 10000 Columns (5), NucleoBond Folded Filters Type 1 (10), NucleoBond Folded Filters Type 2 (10)
• Product Type: PC 10000 Columns
• Purity: A₂₆₀/A₂₈₀: 1.80–1.95
• Size: <300kbp
• Yield: 2,000–10,000μg

Safety and Handling

Hazard Category

• Flammable liquid Category 3
• Substances and mixtures corrosive to metals Category 1
• Skin corrosion/irritation Category 2
• Respiratory sensitiser Category 1
• Serious eye damage/eye irritation Category 2
• Skin sensitiser Category 1