Macherey-Nagel™ NucleoBond™ RNA Soil Mini

Easy handling and superior speed in RNA purification from soil samples for qRT-PCR analysis, as well as parallel preparation of DNA. Complete kit uses anion exchange chromatography and gravity flow columns combined with NucleoSpin Bead Tubes Type A (included).

Brand:  Macherey-Nagel™ 740142.2

Product Code. 15937282

Description

NucleoBond® RNA Soil Mini kit enables efficient isolation of high quality RNA and DNA from any kind of soil or sediment sample. Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, the kit is designed with proven NucleoBond™ technology to enable high sample input and and pure nucleic acids.

• Anion exchange technology to optimize RNA yield and purity—suitable for qRT-PCR analysis
• Combination of mechanical homogenization and chemical lysis allows processing of large samples
• Parallel preparation of RNA and DNA in one hour*
• Sample disruption is achieved with NucleoSpin™ Bead Tubes Type A, optimized Lysis Buffer, and Phenol:Chloroform:Isoamylalcohol
• Optional Buffer OPT enables RNA and DNA purification from mineralic and organic soil samples

* DNA isolation requires the DNA Set for NucleoBond™ RNA Soil Mini, sold separately (man. part no. 740143.10 or 740143.50) or in a combo kit

Specifications

• Format: Gravity Flow Column
• Elution Volume: Elution volume: 50-100 μL
• Efficiency: Binding capacity: 30 μg
• For Use With (Application): RNA/DNA isolation from soil for qRT-PCR
• No. of Reactions: 2
• Purity: A₂₆₀/A₂₈₀: 1.5 - 2.0
• Test Sensitivity: RNA integrity number (RIN): > 7.0
• Yield: 0.25 - 2.5 μg RNA; 1.25 - 12.5 μg DNA

• Isolation Technology: Anion Exchange Chromatography, Gravity Flow Columns, NucleoSpin Bead Tubes
• Sample Type: Soil
• For Use With (Equipment): Centrifuge for microcentrifuge tubes
• Includes: NucleoBond Soil Mini Columns, NucleoSpin Bead Tubes Type A, NucleoSpin RNA columns, Buffers
• Product Type: RNA Kit + DNA Set
• Sample Size: < 0.25 - 0.5 g
• Test Time: 60 min/12 preps (RNA); 15 min/12 preps (DNA)

Safety and Handling

Product Identifier

• NucleoBond RNA Soil Mini + DNA Set (2)

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Supplemental information

• MIXTURE LIST-Contains : guanidium thiocyanide, ethanol