Macherey-Nagel™ NucleoBond™ Xtra Columns

Polypropylene gravity flow columns with inserted column filters for use with NucleoBond Xtra Midi or Maxi kits for ultra-fast isolation of transfection-grade plasmid DNA with high yield.

Product Code	Brand	Size	Sample Volume	Yield
15829097	Macherey-Nagel™ 740410.10S	< 300 kbp	< 200 mL (high copy), < 400 mL (low copy)	400 μg
15749999	Macherey-Nagel™ 740414.10S	< 300 kbp	< 600 mL (high copy), < 1200 mL (low copy)	1000 μg

Description

NucleoBond™ Xtra—The new generation of anion exchanger for ultra-fast transfection-grade plasmid DNA

• Optimized column design – Midi prep in approximately 30 min, Maxi prep in 35 min
• Available in Midi and Maxi sizes – typical yields of 400 μg (Midi) and 1000 μg (Maxi)
• Polypropylene columns suitable for use with organic solvents and buffers with denaturing agents
• Column filter included for high filter flow rates, parallel lysate clearing, and loading onto the column
  • Depth filters avoid time-consuming centrifugation for lysate clearing
  • Complete removal of precipitate even with large lysate volumes without clogging
  • Avoids shearing of large DNA constructs (such as PACs or BACs)

Specifications

• Content And Storage: Room temperature (18 - 25 °C)
• Final Product Type: Transfection grade DNA
• For Use With (Application): Plasmid DNA purification
• Product Type: Gravity Flow Column
• Purity: A₂₆₀/A₂₈₀: 1.80 - 1.95

• Isolation Technology: Anion Exchange Chromatography Technology
• For Use With (Equipment): NucleoBond Xtra
• Includes: NucleoBond Xtra Column Filters
• Quantity: 10 Pack
• Size: < 300 kbp

Safety and Handling

missing translation for 'shelfLife' : At least 1 year

Product Identifier

• NucleoBond Xtra Midi

Supplemental information

• MIXTURE LIST-Contains : 2-propanol/sodium hydroxide solution (diluted < 2 %),dodecylsulfate, sodium salt/ethanol (diluted <