Macherey-Nagel™ NucleoSpin™ 96 RNA Kit

Designed for manual or automated high throughput isolation of RNA from animal or human tissue. Macherey-Nagel™ NucleoSpin™ 96 RNA Kit enables time-saving parallel isolation of total RNA. The kit supplies all core content and convenient accessories for the highest efficiency.

Product Code	Brand	No. of Reactions
12768542	Macherey-Nagel™ 740709.24	24 x 96
12736543	Macherey-Nagel™ 740709.2	2 x 96
12756543	Macherey-Nagel™ 740709.4	4 x 96

Description

NucleoSpin™ 96 RNA kits enable the isolation of RNA from up to 96 samples of fresh, frozen or stabilized animal or human cell cultures. NucleoSpin 96 RNA delivers consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination. The kits combine the stringency of guanidine-isothiocyanate lysis with effective digestion of genomic DNA by DNase I treatment and the speed of silica-membrane technology.

• Time-saving parallel isolation of RNA from cultured cells or from saliva samples collected with Oragene™•RNA
• Suitable for manual processing under vacuum or by centrifugation, or used fully automated on common laboratory workstations
• Includes rDNase for efficient removal of genomic DNA and innovative MN Wash Plate to minimize the risk of cross-contamination
• Produces the highest purity total RNA, suitable for direct use in any kind of enzymatic reaction, such as RT-PCR, TaqMan, Northern Blot or microarray analysis

Kit components: NucleoSpin RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foil, RNase-free rDNase, RNase-free water and all buffers (Lysis RA1; Wash RA2, RA3 and RA4; Reaction for rDNase)

• Sample material: <10⁷ cultured cells (centrifugation), <30mg tissue (centrifugation), saliva (collected with Oragene™)
• Fragment size: >200 nt
• Typical yield: 20μg (2 × 10⁶ HeLa cells), 20μg (20mg mouse liver)
• A₂₆₀/A₂₈₀: 1.90–2.10
• Typical RIN (RNA integrity number): >9 (cells), ≥7 (tissue)
• RNA ratio: 28S/18S approx. 2.1
• Typical concentration: 50–200ng/μL
• Elution volume: 50–130μL
• Preparation time: 70 min/plate
• Binding capacity: 100μg

Specifications

• Concentration: 50–200ng/μL
• Isolation Technology: Silica Membrane
• Sample Type: <1 x 10⁷ Cultured Cells, <30 mg Tissue, Saliva (Collected with Oragene™)
• Product Type: NucleoSpin 96 RNA
• Quantity: 1 Kit
• Yield: 20μg (2 × 10⁶ HeLa cells)
  20μg (20mg mouse liver)

• Format: 96-well Plate
• Elution Volume: Elution volume: 50–130μL
• For Use With (Application): Manual or automated isolation of total RNA from cultured cells and tissue
• Purity: A₂₆₀/A₂₈₀: 1.90–2.10
• Test Time: 70 min/plate

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Respiratory sensitiser Category 1
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P264-Wash with water thoroughly after handling.
• P273-Avoid release to the environment.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : guanidinium thiocyanate/ethanol