Macherey-Nagel™ NucleoSpin™ Plasmid Buffer Set

Used to isolate low-copy plasmids using column chromatography. Macherey-Nagel™ NucleoSpin™ Plasmid Buffer Set is suitable for use only with NuceloSpin™ Plasmid kits. The set includes 75mL of buffers A1 and A2, plus 100mL A3 and RNase A, which is sufficient for 300 additional preps.

Brand:  Macherey-Nagel™ 740953

Product Code. 11962562

Description

NucleoSpin™ Plasmid Kit is used for the isolation of low-copy plasmid DNA using a time-saving parallel process. It is suitable for manual or automated processing under vacuum or by centrifugation. It can process up to 5mL of E. coli culture, with a typical yield of 4–6μg of sequencing-grade plasmid DNA per 1mL sample culture.

• NucleoSpin™ Plasmid kits include filter and binding plates/strips, buffers and RNase A
• When a larger amount of low-copy plasmid is required, larger culture volumes can be prepared using additional buffer volumes
• Plasmid Buffer Set provides: 75mL of Resuspension Buffer A1 and Lysis Buffer A2, plus 100mL of Neutralization Buffer A3 and RNase A
• Suitable for use only with NucleoSpin™ Plasmid kits—Sufficient for up to 300 preps

Specifications

• Description: 75mL of Resuspension Buffer A1 and Lysis Buffer A2, plus 100mL of Neutralization Buffer A3 and RNase A
• Quantity: 1 Set
• Buffer: Resuspension Buffer A1, Lysis Buffer A2, Neutralization Buffer A3

• For Use With (Equipment): NucleoSpin™ Plasmid Kit
• For Use With (Application): Isolation of low-copy plasmid DNA

Safety and Handling

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), guanidine hydrochloride, RNase