Macherey-Nagel™ NucleoSpin™ Plasmid Columns

Replacement columns for use with NucleoSpin™ Plasmid kits for rapid, small-scale preparation of high-purity, sequencing-grade plasmid DNA.

Product Code	Brand	Quantity
15870928	Macherey-Nagel™ 740588.50S	50 Each
15860928	Macherey-Nagel™ 740588.250S	250 Each

Description

NucleoSpin™ Plasmid

• For rapid small scale preparation high-purity plasmid DNA
• Fast procedure – 6 Mini preps in 25 min
• High capacity – up to 50 μg of plasmid DNA
• Chaotropic salt wash buffer – extra pure DNA
• Downstream applications: cloning, sequencing, PCR, transformation, restriction analysis
  • High-copy plasmids from E. coli
  • Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required)
  • Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction mixtures
  • Supplementary protocol for M13 DNA availabl

Specifications

• Color: Clear
• Isolation Technology: Silica Membrane Technology
• Efficiency: Binding capacity: 50 μg
• Final Product Type: Sequencing-grade plasmid DNA
• For Use With (Application): Preparation of high-purity plasmid DNA
• Product Type: NucleoSpin Plasmid lidded Column

• Format: Mini Spin Column
• Sample Type: E. coli Culture
• Elution Volume: Elution volume: 50 μL
• For Use With (Equipment): NucleoSpin™ Plasmid Kit
• Includes: Columns only
• Sample Volume: 2 - 10 mL

Safety and Handling

Product Identifier

• NucleoSpin Plasmid

Supplemental information

• MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/2-pr