Macherey-Nagel™ Nucleospin™ Plasmid Transfection-grade

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.

Brand:  Macherey-Nagel™ 740490.250

Product Code. 15593626

Description

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

• Purifies high-copy plasmids for transfection
• Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
• Rapid purification from E. coli via manual, vacuum or centrifugation processing
• Ultra-fast procedure using mini spin columns simplifies the work-flow
• Downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

• Bacterial Endotoxins: <50 EU/ng DNA
• Format: Mini Spin Column
• Sample Type: Bacterial Culture (E. coli, High-copy Plasmids)
• Elution Volume: Elution Volume: 30 - 50 μL
• For Use With (Equipment): Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
• Includes: NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A
• Purity: A₂₆₀/A₂₈₀: 1.80 - 1.85
• Size: < 15 kbp
• Yield: 15 - 30 μg

• Content And Storage: Room temperature (18 - 25 °C)
• Isolation Technology: Silica Membrane and Endotoxin-reduction Technology
• Efficiency: Binding capacity: 35 μg
• Final Product Type: Transfection-grade plasmid DNA
• For Use With (Application): Rapid purification of plasmids for transfection
• No. of Samples: 250
• Sample Volume: Up to 5 mL
• Test Time: 14 min/6 preps

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Specific target organ toxicity Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/trie