Macherey-Nagel™ NucleoSpin™ RNA Columns

Additional silica-membrane mini spin columns for use in isolating RNA of the highest integrity from cells and tissue using the NucleoSpin™ RNA kit.

Brand:  Macherey-Nagel™ 740955.250S

Product Code. 15870968

Description

NucleoSpin™ RNA

• rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready to use RNA
• Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
• RNA isolation from cultured cells, tissue (standard protocol):
  • RNA from < 10⁹ bacterial cells (Gram-negative, Gram-positive) or < 10⁸ yeast cells
  • RNA from ≤ 100 μL biological fluids
  • RNA clean-up from reaction mixtures
  • RNA from samples stored in RNAlater™
  • RNA from saliva samples collected with Oragene™ RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

• Color: Blue band
• Format: Mini Spin Column
• Elution Volume: 40 - 120 μL
• Efficiency: Binding capacity: 200 μg
• For Use With (Application): Isolation of RNA from cells and tissue
• Quantity: 250 Columns
• Test Time: 35 min/6 preps

• Content And Storage: rDNase at 4 °C; Other components at room temperature (18 - 25 °C)
• Isolation Technology: Silica Membrane
• Sample Type: Cultured Bacterial or Yeast Cells, Human or Animal Tissue
• For Use With (Equipment): NucleoSpin RNA Kits
• Includes: Columns only
• Sample Size: < 30 mg tissue, 5 x 10⁶ cultured cells

Safety and Handling

Product Identifier

• NucleoSpin RNA

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Flammable liquid Category 3
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 1 B
• Skin corrosion/irritation Category 2

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); gu