Macherey-Nagel™ NucleoSpin™ RNA, Mini Kit for RNA purification

Mini spin kit for isolation of RNA of highest integrity.

Product Code	Brand	No. of Reactions	Format
15220023	Macherey-Nagel™ 740955.240C	240	QIAcube
12373368	Macherey-Nagel™ 740955.250	250	Mini Spin Column
11922402	Macherey-Nagel™ 740955.50	50	Mini Spin Column
11912512	Macherey-Nagel™ 740955.10	10	Mini Spin Column

Description

Features

• DNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin™ Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set

Applications

• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater™
• RNA from saliva samples collected with Oragene™ RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

• Bacterial Content: 70 μg
• Isolation Technology: Silica Membrane
• Final Product Type: NucleoSpin™ RNA Kit
• Product Type: NucleoSpin™ RNA Kit

• Detection Method: Silica-membrane
• DNase: Yes
• For Use With (Application): RNA isolation from cultured cells, tissue
• Quantity: 1 Each

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Respiratory sensitiser Category 1
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) / guanidine hydrochloride, ethanol