Macherey-Nagel™ NucleoSpin™ RNA Plus

State-of-the-art, dual-column kit includes gDNA removal columns and RNA binding columns for efficient and quick RNA isolation from cells and tissues.

Brand:  Macherey-Nagel™ 740984.250

Product Code. 15300205

Description

NucleoSpin™ RNA Plus

• Efficient and quick RNA isolation from cells and tissues
• Two-column system includes gDNA removal columns and RNA binding columns
• NucleoSpin™ gDNA Removal Columns – no time consuming rDNase digestion
• Convenient handling – lysate clearing and gDNA removal on one column in one step
• New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary
• Variety of sample materials – cultured cells, animal tissue, plant tissue, microorganisms
• Downstream applications include real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

• Color: Yellow/Blue ring
• Format: Mini Spin Column
• Elution Volume: 30 - 120 μL
• Efficiency: Binding capacity: 200 μg
• For Use With (Equipment): Centrifuge
• Includes: NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers
• Product Type: RNA Isolation Kit
• Sample Size: < 30 mg tissue; < 30 mg tissue
• Yield: HeLa cells (5 x 10⁶): 40-60 μg
  Mouse liver (20 mg): 80-100 μg
  Mouse kidney (20 mg): 40-70 μg
  Mouse spleen (5 mg): 30-60 μg

• Content And Storage: Room temperature (18-25 °C)
• Isolation Technology: Silica Membrane
• Sample Type: Cultured Bacterial or Yeast Cells, Human or Animal Tissue
• Final Product Type: RNA (with typical RIN > 9)
• For Use With (Application): Isolation of RNA
• No. of Reactions: 250 Preps
• Purity: A₂₆₀/A₂₈₀: 1.9 - 2.1
• Test Time: 20 min/6 preps

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Supplemental information

• MIXTURE LIST-Contains : ethyleneglycol diacetate, guanidinium thiocyanate, guanidinehydrochloride, ethanol