Macherey-Nagel™ NucleoSpin™ RNA Plus Kit

Efficient and quick RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms. Macherey-Nagel™ NucleoSpin™ RNA Plus Kit includes the NucleoSpin™ gDNA Removal Column, a spin column, which quickly and effectively removes genomic DNA contamination without DNase digestion.

Brand:  Macherey-Nagel™ 740984.10

Product Code. 15370195

Description

During the isolation of RNA, it is imperative to prevent the degradation of the RNA. In the NucleoSpin™ RNA Plus method, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases. The lysate is added to the gDNA Removal Column to clarify the lysate and to remove contaminating gDNA. After the addition of the Binding Solution to the flow-through, the RNA is bound to the RNA Plus Column. Two subsequent wash steps remove salts, metabolites, and macromolecular cellular components. High quality RNA is eluted with RNase-free H₂O.

• Includes NucleoSpin™ gDNA Removal Column: no time consuming rDNase digestion
• Convenient handling: lysate clearing and gDNA removal with one column in one step
• New efficient lysis buffer: no β-mercaptoethanol or TCEP necessary
• Downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
• Kit contents: NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes, buffers

Specifications

• Bacterial Content: <10⁹ Cells
• Buffer: Lysis Buffer
• Isolation Technology: Silica Membrane System (Two Columns)
• Sample Type: <1 x 10⁷ Cultured Cells, <1 x 10⁹ Bacterial Cells, <1 x 10⁸ Yeast Cells, <30 mg Human/Animal Tissue
• Includes: NucleoSpin™ RNA Plus Columns with Collection Tubes, NucleoSpin™ gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers
• Product Type: NucleoSpin RNA Plus kit
• RNase: Yes
• Test Time: 20 min./6 Reaction

• Content And Storage: Room temperature (18-25°C)
• Format: Mini Spin Column
• Elution Volume: Elution volume: 30–120μL
• For Use With (Application): RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• No. of Reactions: 10
• Quantity: 1 Kit
• Size: Fragment size: >200 nt
• Yield: HeLa cells 5 x 10⁶: 40-60μg
  Mouse liver 20mg: 80-100μg
  Mouse kidney 20mg: 40-70μg
  Mouse spleen 5mg: 30-60μg

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H412-Harmful to aquatic life with long lasting effects.

Supplemental information

• MIXTURE LIST-Contains : ethyleneglycol diacetate / guanidinium thiocyanate /guanidine hydrochloride, ethanol